Table 4.

Equilibrium substrate concentrations and enzyme saturation levels for maximum unidirectional reaction rates achieved with various binding affinities.

ΔGBindingo(RTln(10))[A]1 = [A]2 (M)log[A]1 = log[A]2 (M)Rmax+=Rmax(pmol cm2s1)saturation (%)
110.0010155151.0000441011.24987501650.002538661
01.0010002940.0004342051.24875156050.024994849
−10.100994898−0.9957005671.23765409550.247493345
−20.010954431−1.9604101951.13872124752.277396600
−30.001732047−2.7614404520.66987298163.397408613
−40.000458256−3.3388923130.16043560782.087071165
−50.000141771−3.8484141280.02170563893.411125972
−60.000044728−4.3494253750.00239066897.813156053