Table 3.

Equilibrium substrate concentrations and enzyme saturation levels for maximum equilibrium unidirectional reaction rates (Rmax+=Rmax) achieved with various binding affinities.

log[A]1 = log[A]2 (M)ΔGBindingo(RTln(10))Rmax+=Rmax(pmol cm2s1)saturation (%)
11.0000441131.24987501649.997460652
00.0004341061.24875156049.975010855
−1−0.9957005281.23765409549.752504512
−2−1.9604130651.13872124747.722614266
−3−2.7614406320.66987298136.602607666
−4−3.3388925510.16043560817.912952906
−5−3.8484126680.0217056386.588875043
−6−4.3494079130.0023906692.186781851