Table 5.

Estimate of virtual ‘Km’ for cis-substrate concentration [A]1 as influenced by rate limitation of reaction step 4 and trans-substrate concentration [A]2.

k4 = k−4 (s−1)Rmax+(pmolcm2s1)[A]2 (mM)[A]1Km’ (mM)log[A]1Km’ (M)saturation (%)
1040.450510010.9910−1.959072.3379
2.38101010.9524−1.960551.1388
4.1667110.9167−1.959031.4568
1030.04731005.4167−2.266352.8773
0.3205104.3590−2.360638.3861
0.757612.6667−2.574013.8258